Journal: eLife

Article Title: A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells

doi: 10.7554/eLife.50999

Figure Lengend Snippet: Freshly seeded Vero E6/7 (African green monkey kidney), SW13 (Human Adrenal gland cortical small cell carcinoma), HAE/CTVM8 ( Hyalomma anatolicum embryo) and BDE/CTVM16 ( Rhipicephalus (Boophilus) decoloratus embryo) cells were inoculated with the supernatant of CCHFV-positive ticks and CCHFV-positive pools (MT-P-1302–1311 and MT-P-1358–1367). Eight days post-infection, supernatants of cell lines were passaged onto fresh cells. Before passaging, CCHFV RNA was measured in supernatants by qRT-PCR. Infected tick cells were observed for a period of 70 days.

Article Snippet: Freshly seeded 4 × 10 4 Vero E6/7 (African green monkey kidney, ATCC, cell identity has been authenticated by STR profiling by ATCC), 4 × 10 4 SW13 (Human Adrenal gland cortical small cell carcinoma, gift from DA Bente, University of Texas Medical Branch, originally obtained from ATCC, cell identity has been authenticated by STR profiling by ATCC), 2.5 × 10 5 HAE/CTVM8 ( Hyalomma anatolicum embryo, Tick Cell Biobank, University of Liverpool, cell identity has been authenticated by Tick Cell Biobank [ ]) and 1.8 × 10 5 BDE/CTVM16 ( Rhipicephalus (Boophilus) decoloratus embryo, Tick Cell Biobank, University of Liverpool, cell identity has been authenticated by Tick Cell Biobank [ ]) cells in 48-well-plates were inoculated with the supernatant of CCHFV-positive ticks and CCHFV-positive pools filtrated through a 0.45 μm filter under S3 laboratory conditions.

Techniques: Infection, Passaging, Quantitative RT-PCR